Coding

Part:BBa_K209412:Design

Designed by: Katja Kolar   Group: iGEM09_UCSF   (2009-09-30)

AarI C-D part, Lamellipodin


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1967
    Illegal PstI site found at 2687
    Illegal PstI site found at 3062
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1967
    Illegal PstI site found at 2687
    Illegal PstI site found at 3062
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 157
    Illegal BamHI site found at 1496
    Illegal BamHI site found at 1609
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1967
    Illegal PstI site found at 2687
    Illegal PstI site found at 3062
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1967
    Illegal PstI site found at 2687
    Illegal PstI site found at 3062
    Illegal AgeI site found at 2568
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1302
    Illegal BsaI site found at 3032
    Illegal BsaI.rc site found at 2979
    Illegal BsaI.rc site found at 3046
    Illegal BsaI.rc site found at 3719


Design Notes

Cloned into pCR2.1.

Source

References